Part:BBa_K4186003

Gold Inducer

General Information

It induces the production of a colour in the presence of gold.

Usage and Biology

The golTSB from the Salmonella enterica serovar typhimurium is regulated by Au ions to provide gold resistance to the bacteria. In one experiment, this regulon was engineered by attaching a promoterless reporter gene lacZ “downstream of the golB open frame as a transcriptional fusion” (i.e. golTSB::lacZ)[1]. Recent research has shown that GolS promotes the transcription of the golTS operon and golB, a tiny neighboring gene that codes for a putative metal-binding protein when there are Au ions present [2]. golB is a gold binding protein to ensure that gold ions do not move freely in the cell.

Also, there are regulatory promoters (PgolS & PgolB) controlled by golS. In order words, golS induces transcription from these promoters once golS is activated upon binding a gold ion [3], illustrated in the figure above.

PgolTS works as a constitutive promoter ensuring the production of golS protein, in the presence of Au+, the golS binds with the gold ions stopping the inhibition of PgolB, hence transcription. The reporter gene is expressed by attaching a promoterless amilCP to produce that blue colour even as the golS proteins bind with gold ions in the cytoplasm.

References

[1] C. M. Zammit, D. Quaranta, A. J. Z. Shane gibson, J. B. Christine ta, R. y. Lai, g. Grass and F. Reith, "A whole-cell Biosensor for the Detection of Gold," Plos One, vol. 8, no. 8, 2013.

[2] S. K. Checa, M. Espariz, M. E. P. Audero, P. E. Botta, F. C. Soncini and S. V. Spinelli, "Bacterials sensing of and resistance to gold salts," Molecular Microbiology, vol. 63, no. 5, pp. 1307-1318, January 2007.

[3]T.Hsiao,"iGEMParts,"26 October 2010. Available: https://parts.igem.org/Part:BBa_K310009.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 548
  
• 1000
  COMPATIBLE WITH RFC[1000]